Get effective polyclonal antisera in one month
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چکیده
منابع مشابه
Monoclonal and polyclonal antisera
The extensive application of the hybridoma technology for tissue phenotyping has demonstrated in the last decade that a number of heterodimeric molecules consisting of noncovalently associated α and β subunits (Hynes, 1992), named integrins, play a major role in cell-to-cell and cellto-interstitium interactions as well as in cell trafficking (Hemler, 1990; Albelda and Buck, 1990). The structura...
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There has been an overall increase in the incidence of multiple pregnancies and assisted reproduction technology is largely responsible for this rise. Although twins may appeal to couples undergoing in vitro fertilisation (IVF), they have been associated with serious health consequences to the babies, their mothers and the family unit, as well as having massive financial implications for the Na...
متن کاملComparison of polyclonal rabbit antisera with monoclonal antibodies for serological typing of Pseudomonas aeruginosa.
A panel of 219 distinct strains of Pseudomonas aeruginosa were serotyped with a set of monoclonal antibodies prepared against the serotype strains of the Homma scheme (J. Y. Homma, Jpn. J. Exp. Med. 46:329-336, 1976). A total of 87.6% were typable, and there was very good agreement with the corresponding polyclonal serotype. A high proportion of strains that were polyagglutinating or nontypable...
متن کاملNegative purification method for the selection of specific antibodies from polyclonal antisera.
We developed a protocol to remove non-specific antibodies from polyclonal antisera by adsorption on non-target antigens immobilized on nitrocellulose membranes. This "negative" purification method is simple and provides better immunoreagents than the blocking of nonspecific antibodies in solution or the enrichment of specific antibodies on nitrocellulose membranes. For routine applications, thi...
متن کاملParathyroid-hormone-related peptide immunochemiluminometric assay. Developed with polyclonal antisera produced from a single animal.
An immunochemiluminometric assay of parathyroid-hormone-related peptide (PTHrP) was developed with purified antisera produced from a single goat immunized with (1-86) PTHrP. One batch of purified antibodies was labeled with acridinium ester used as tracer antibodies; a second batch of the purified antibodies was immobilized onto plastic bead. Sensitivity of the assay was 0.1 pmol/L. The assay h...
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ژورنال
عنوان ژورنال: Cell Research
سال: 2002
ISSN: 1001-0602,1748-7838
DOI: 10.1038/sj.cr.7290122